Natural Mutations in a 2‘−5‘ Oligoadenylate Synthetase Transgene Revealed Residues Essential for Enzyme Activity†
Identifieur interne : 002F29 ( Main/Exploration ); précédent : 002F28; suivant : 002F30Natural Mutations in a 2‘−5‘ Oligoadenylate Synthetase Transgene Revealed Residues Essential for Enzyme Activity†
Auteurs : Saumendra N. Sarkar [États-Unis] ; Sean P. Kessler [États-Unis] ; Theresa M. Rowe [États-Unis] ; Mitali Pandey [États-Unis] ; Arundhati Ghosh [États-Unis] ; Christopher P. Elco [États-Unis] ; Rune Hartmann [États-Unis] ; Srabani Pal [États-Unis] ; Ganes C. Sen [États-Unis]Source :
- Biochemistry [ 0006-2960 ] ; 2005.
Abstract
Unlike other RNA polymerases, 2‘−5‘ oligoadenylate synthetases, a family of interferon-induced enzymes, catalyze the formation of 2‘−5‘, not 3‘−5‘, phosphodiester bonds. Moreover, to be active, these proteins require double-stranded RNA as a cofactor. We have been identifying the specific residues of these proteins that impart their novel properties. Here, we report the identity of three such residues that underwent natural mutations in a transgenic mouse line. When deliberately introduced into recombinant proteins, each of these mutations rendered the protein enzymatically inactive. In an effort to understand the roles of these residues in enzyme activity, new mutants carrying other residues in one of these three sites were generated. Detailed characterization of the properties of the mutant proteins revealed that Lys 404 is needed for proper binding of the acceptor substrate, Pro 500 provides structural flexibility to the protein, and Ser 471 is probably required for its proper folding. This study illustrates the power of using natural mutations in transgenes as guides for studying structure−function relationships of proteins.
Url:
DOI: 10.1021/bi0502893
Affiliations:
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Residues Essential for Enzyme Activity<ref type="bib" target="#bi0502893AF2">†</ref></title><author><name sortKey="Sarkar, Saumendra N" sort="Sarkar, Saumendra N" uniqKey="Sarkar S" first="Saumendra N." last="Sarkar">Saumendra N. Sarkar</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Molecular Biology, The Lerner Research Institute, Cleveland Clinic Foundation, Cleveland</wicri:cityArea></affiliation></author><author><name sortKey="Kessler, Sean P" sort="Kessler, Sean P" uniqKey="Kessler S" first="Sean P." last="Kessler">Sean P. Kessler</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Molecular Biology, The Lerner Research Institute, Cleveland Clinic Foundation, Cleveland</wicri:cityArea></affiliation></author><author><name sortKey="Rowe, Theresa M" sort="Rowe, Theresa M" uniqKey="Rowe T" first="Theresa M." last="Rowe">Theresa M. Rowe</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Molecular Biology, The Lerner Research Institute, Cleveland Clinic Foundation, Cleveland</wicri:cityArea></affiliation></author><author><name sortKey="Pandey, Mitali" sort="Pandey, Mitali" uniqKey="Pandey M" first="Mitali" last="Pandey">Mitali Pandey</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Molecular Biology, The Lerner Research Institute, Cleveland Clinic Foundation, Cleveland</wicri:cityArea></affiliation></author><author><name sortKey="Ghosh, Arundhati" sort="Ghosh, Arundhati" uniqKey="Ghosh A" first="Arundhati" last="Ghosh">Arundhati Ghosh</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Molecular Biology, The Lerner Research Institute, Cleveland Clinic Foundation, Cleveland</wicri:cityArea></affiliation></author><author><name sortKey="Elco, Christopher P" sort="Elco, Christopher P" uniqKey="Elco C" first="Christopher P." last="Elco">Christopher P. Elco</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Molecular Biology, The Lerner Research Institute, Cleveland Clinic Foundation, Cleveland</wicri:cityArea></affiliation></author><author><name sortKey="Hartmann, Rune" sort="Hartmann, Rune" uniqKey="Hartmann R" first="Rune" last="Hartmann">Rune Hartmann</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Molecular Biology, The Lerner Research Institute, Cleveland Clinic Foundation, Cleveland</wicri:cityArea></affiliation></author><author><name sortKey="Pal, Srabani" sort="Pal, Srabani" uniqKey="Pal S" first="Srabani" last="Pal">Srabani Pal</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Molecular Biology, The Lerner Research Institute, Cleveland Clinic Foundation, Cleveland</wicri:cityArea></affiliation></author><author><name sortKey="Sen, Ganes C" sort="Sen, Ganes C" uniqKey="Sen G" first="Ganes C." last="Sen">Ganes C. Sen</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Molecular Biology, The Lerner Research Institute, Cleveland Clinic Foundation, Cleveland</wicri:cityArea></affiliation><affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Biochemistry</title><title level="j" type="abbrev">Biochemistry</title><idno type="ISSN">0006-2960</idno><idno type="eISSN">1520-4995</idno><imprint><publisher>American Chemical Society</publisher><date type="e-published">2005</date><date type="published">2005</date><biblScope unit="vol">44</biblScope><biblScope unit="issue">18</biblScope><biblScope unit="page" from="6837">6837</biblScope><biblScope unit="page" to="6843">6843</biblScope></imprint><idno type="ISSN">0006-2960</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0006-2960</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">Unlike other RNA polymerases, 2‘−5‘ oligoadenylate synthetases, a family of interferon-induced enzymes, catalyze the formation of 2‘−5‘, not 3‘−5‘, phosphodiester bonds. Moreover, to be active, these proteins require double-stranded RNA as a cofactor. We have been identifying the specific residues of these proteins that impart their novel properties. Here, we report the identity of three such residues that underwent natural mutations in a transgenic mouse line. When deliberately introduced into recombinant proteins, each of these mutations rendered the protein enzymatically inactive. In an effort to understand the roles of these residues in enzyme activity, new mutants carrying other residues in one of these three sites were generated. Detailed characterization of the properties of the mutant proteins revealed that Lys 404 is needed for proper binding of the acceptor substrate, Pro 500 provides structural flexibility to the protein, and Ser 471 is probably required for its proper folding. This study illustrates the power of using natural mutations in transgenes as guides for studying structure−function relationships of proteins.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Ohio</li></region></list><tree><country name="États-Unis"><region name="Ohio"><name sortKey="Sarkar, Saumendra N" sort="Sarkar, Saumendra N" uniqKey="Sarkar S" first="Saumendra N." last="Sarkar">Saumendra N. Sarkar</name></region><name sortKey="Elco, Christopher P" sort="Elco, Christopher P" uniqKey="Elco C" first="Christopher P." last="Elco">Christopher P. Elco</name><name sortKey="Ghosh, Arundhati" sort="Ghosh, Arundhati" uniqKey="Ghosh A" first="Arundhati" last="Ghosh">Arundhati Ghosh</name><name sortKey="Hartmann, Rune" sort="Hartmann, Rune" uniqKey="Hartmann R" first="Rune" last="Hartmann">Rune Hartmann</name><name sortKey="Kessler, Sean P" sort="Kessler, Sean P" uniqKey="Kessler S" first="Sean P." last="Kessler">Sean P. Kessler</name><name sortKey="Pal, Srabani" sort="Pal, Srabani" uniqKey="Pal S" first="Srabani" last="Pal">Srabani Pal</name><name sortKey="Pandey, Mitali" sort="Pandey, Mitali" uniqKey="Pandey M" first="Mitali" last="Pandey">Mitali Pandey</name><name sortKey="Rowe, Theresa M" sort="Rowe, Theresa M" uniqKey="Rowe T" first="Theresa M." last="Rowe">Theresa M. Rowe</name><name sortKey="Sen, Ganes C" sort="Sen, Ganes C" uniqKey="Sen G" first="Ganes C." last="Sen">Ganes C. Sen</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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